Table of Contents

1. Introduction
2. Diagnostic Methods for Rotavirus Infection
3. Clinical Symptoms
4. Laboratory Tests
5. QL Biotech Company Solutions
6. References

Introduction

Rotavirus is a leading cause of severe diarrhea among infants and young children worldwide. Accurate diagnosis is crucial for managing and controlling outbreaks. This article outlines the methods used to diagnose rotavirus infection, highlighting laboratory techniques and innovative solutions provided by biotech companies such as QL Biotech.

Diagnostic Methods for Rotavirus Infection

Diagnosing rotavirus infection involves a combination of clinical assessment and laboratory tests. The primary goal is to confirm the presence of the virus in stool samples.

Clinical Symptoms

Rotavirus infection typically presents with symptoms such as severe watery diarrhea, vomiting, fever, and abdominal pain. These symptoms appear approximately 2 days after exposure and can last up to 8 days. However, clinical diagnosis based solely on symptoms is unreliable due to overlaps with other viral gastroenteritis.

Laboratory Tests

Laboratory testing is the definitive method for diagnosing rotavirus infection. The following are key laboratory techniques used:

• Enzyme-Linked Immunosorbent Assay (ELISA): This test detects rotavirus antigens in stool samples. With a sensitivity of 90%, it is widely used in clinical laboratories.
• Polymerase Chain Reaction (PCR): A molecular technique that amplifies rotavirus RNA, providing a sensitivity of over 95%. PCR is advantageous for its ability to differentiate rotavirus strains.
• Rapid Antigen Detection Tests: These immunochromatographic assays can give results within 15 minutes. They are practical for quick diagnosis in clinical settings, although they may have lower sensitivity compared to ELISA and PCR.

QL Biotech Company Solutions

QL Biotech has developed innovative diagnostic kits that enhance the accuracy and speed of rotavirus detection. Their products include:

• QL-Rotavirus ELISA Kit: Featuring enhanced sensitivity (95%) and specificity (98%), this kit offers reliable detection of rotavirus antigens within 2 hours.
• QL-Rotavirus qPCR Kit: This real-time PCR kit allows for high-throughput screening, with a limit of detection as low as 10 RNA copies per reaction.
• QL Rapid Test: Designed for point-of-care testing, this rapid antigen test yields results in 10 minutes, maintaining a sensitivity of 85% and specificity of 95%.

References

1. Jiang, B., & Greenberg, H. (2001). Diversity and evolution of rotaviruses. In Virus Res, 13(1):67-80.
2. Parashar, U.D., & Glass, R.I. (2009). Rotavirus vaccines--early success, remaining questions. N Engl J Med, 360(11):1063-5.
3. World Health Organization. (2017). Global rotavirus surveillance: 2017 report.
4. QL Biotech Company. (2023). Product Catalog and Specifications. Available at: www.qlbiotech.com/products