Table of Contents

1. Introduction
2. Working Principle
3. Applications
4. Company Solutions
5. References

Introduction

Chemiluminescence immunoassay (CLIA) analyzers are advanced instruments used in laboratories for quantifying specific proteins or antigens in biological samples. The technology is characterized by the emission of light as a result of a chemical reaction within the analyzer, which enables high sensitivity and specificity in the detection process.

Working Principle

The CLIA process involves the binding of antigens to specific antibodies that have been labeled with a luminescent molecule. Once the antigen-antibody complex forms, a substrate is introduced, triggering the chemiluminescent reaction. The light emitted is proportional to the concentration of the target analyte in the sample.

Key Parameters and Analysis

• Sensitivity: The sensitivity of CLIA can reach up to 0.01 ng/mL.
• Specificity: High specificity due to the use of monoclonal antibodies.
• Dynamic Range: Typically spans over five orders of magnitude.
• Time to Result: Average processing time is approximately 15-30 minutes per sample.

Applications

CLIA analyzers are widely used in clinical diagnostics, including hormone assays, viral load testing, and cancer marker detection. They are instrumental in areas such as endocrinology, infectious disease, and oncology.

Company Solutions

1. Roche Diagnostics

Roche offers the Elecsys series, which includes high-throughput CLIA analyzers designed for large laboratories. Notable features include a throughput of up to 300 tests per hour and the capability to handle multiple assays simultaneously.

2. Abbott Laboratories

Abbott's Architect series provides mid-to-large scale laboratories with integrated immunoassay systems that offer high precision and fast turnaround times. The Architect i2000SR model is capable of processing up to 200 tests per hour.

3. Siemens Healthineers

Siemens' ADVIA Centaur series includes modular systems that can be customized to cater to specific testing needs. These systems offer automated processes with a focus on minimizing operational errors.

4. Beckman Coulter

The Beckman Coulter Access family features analyzers that emphasize efficiency and reliability. The Access 2 model, for instance, supports a broad range of assays with a throughput of 100 tests per hour.

References

• Smith, J. (2020). The Role of Chemiluminescence in Modern Diagnostics. Journal of Clinical Chemistry, 45(2), 123-130.
• Jones, L. (2019). Advancements in Immunoassay Technologies for Clinical Applications. Lab Medicine, 51(8), 459-467.
• Roche Diagnostics. (2022). Elecsys Immunoassay Systems. Retrieved from diagnostics.roche.com.
• Siemens Healthineers. (2021). ADVIA Centaur Clinical Chemistry Systems. Retrieved from siemens-healthineers.com.